Avermectins/ Ivermectin ELISA Test Kit
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| Post Date: | Sep 10,2015 |
| Expiry Date: | Sep 09,2016 |
| Detailed Description: |
Product Description
REAGEN? Avermectins/ Ivermectin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Abamectin in meat, animal tissue, Vegetables and fruit., The unique features of the kit are: The extraction of Avermectins from milk can be finished with a high recovery rate of 75 - 95%. A quick ELISA assay (less than 2 hours regardless of number of samples). High reproducibility. Procedure Overview The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample. Kit Contents, Storage and Shelf Life REAGEN? Avermectins/ Ivermectin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C *. The shelf life is 12 months when the kit is properly stored. Kit Contents Amount Storage Avermectin-coated Microtiter Plate 1 x 96-well plate (8 wells x 12 strips) 2-8C Ivermectin Standards: Negative control 0.5 ng/mL 1.0 ng/mL 2 ng/mL 4 ng/mL 8 ng/mL 1200 ng/mL (spiking, optional) 0.8 mL 2-8C * Avermectin Antibody #1 12ml 2-8C * 100X HRP-Conjugated Antibody #2 250 L 2-8C * Antibody #2 Diluent 20 mL 2-8C 10X Sample Extraction Buffer 25 mL 2-8C 20X Wash Solution 28 mL 2-8C Stop Buffer 14 mL 2-8C TMB Substrate 12 mL 2-8C Avermectin Clean Up Mix 25g 2-8C Milk Extraction Buffer I 10 mL 2-8C Milk Extraction Buffer II 28 mL 2-8C * If you are not planning to use the kit for over 1 month, store Ivermectin Standard , Avermectin Antibody #1, 100X HRP-Conjugated Antibody #2 at -20C preferably or in a freezer. Sensitivity |
| Company: | REAGEN LLC [ United States ] |
| Contact: | Nicole |
| Tel: | 8568585434 |
| Fax: | 1-302-269-1897 |
| Email: | reagenkits@gmail.com |
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